prkcb monoclonal antibody  (Proteintech)

Journal: iScience

Article Title: Downregulation of protein kinase C gamma reduces epithelial property and enhances malignant phenotypes in colorectal cancer cells

doi: 10.1016/j.isci.2022.105501

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Article Snippet: Mouse monoclonal anti-PKCγ for B–5D , Santa Cruz , Cat#sc-166385, RRID: AB_2018059.

Techniques: Recombinant, Reporter Assay, Expressing, Negative Control, Plasmid Preparation, Software

Journal: Cell

Article Title: Metabolic Fingerprinting Links Oncogenic PIK3CA with Enhanced Arachidonic Acid-Derived Eicosanoids

doi: 10.1016/j.cell.2020.05.053

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Article Snippet: Mouse monoclonal anti-PKCzeta (B-7) , Santa Cruz Biotechnology , Cat# sc-393218.

Techniques: Produced, Virus, Recombinant, Transfection, Protease Inhibitor, Lysis, Mutagenesis, Proliferation Assay, In Situ, Calcium Assay, Reverse Transcription, Kinase Assay, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Proximity Ligation Assay, CRISPR, Control, shRNA, Amplification, Plasmid Preparation, Positive Control, Software, Modification, Targeted Proteomics, Mass Spectrometry, Western Blot

Journal: Oncotarget

Article Title: Identification of miR-34a-target interactions by a combined network based and experimental approach

doi: 10.18632/oncotarget.9103

Figure Lengend Snippet: The location of the predicted binding sites of miR-34a-5p in the 3′UTRs of PRKCA , PRKCB , PRKCE , PRKCH , PRKCQ and additionally the sequences of the binding sites of miR-34a-5p as well as the mutated binding sites (underlined capital letters) are shown. ( A ) PRKCA -3′UTR, ( B ) PRKCB -3′UTR, ( C ) PRKCE -3′UTR, ( D ) PRKCH -3′UTR and ( E ) PRKCQ -3′UTR.

Article Snippet: The primary antibodies used were anti-PRKCA polyclonal rabbit antibody (2056S, Cell Signaling Technology, Danvers, United States), anti-PRKCB monoclonal mouse antibody (ABIN967769, antibodies-online GmbH, Aachen, Germany), anti-PRKCQ monoclonal rabbit antibody (E1I7Y, Cell Signaling Technology, Danvers, United States) and anti-β-actin monoclonal mouse antibody (AC-15, Sigma Aldrich, Munich, Germany).

Techniques: Binding Assay

Journal: Oncotarget

Article Title: Identification of miR-34a-target interactions by a combined network based and experimental approach

doi: 10.18632/oncotarget.9103

Figure Lengend Snippet: PRKCA , PRKCB, PRKCE, PRKCH and PRKCQ . HEK 293T cells were transfected with empty vectors, reporter gene constructs and miRNA-expression plasmids in the indicated combinations. The luciferase activity of the control vector experiment was set to 100%. The results represent the mean of at least three independent experiments carried out in duplicates. Three asterisks correspond to a P value lower than 0.001. Data are represented as mean +/− SEM. ( A ) PRKCA -3′UTR, ( B ) PRKCB -3′UTR, ( C ) PRKCE -3′UTR, ( D ) PRKCH -3′UTR and ( E ) PRKCQ -3′UTR.

Article Snippet: The primary antibodies used were anti-PRKCA polyclonal rabbit antibody (2056S, Cell Signaling Technology, Danvers, United States), anti-PRKCB monoclonal mouse antibody (ABIN967769, antibodies-online GmbH, Aachen, Germany), anti-PRKCQ monoclonal rabbit antibody (E1I7Y, Cell Signaling Technology, Danvers, United States) and anti-β-actin monoclonal mouse antibody (AC-15, Sigma Aldrich, Munich, Germany).

Techniques: Transfection, Construct, Expressing, Luciferase, Activity Assay, Plasmid Preparation

Journal: Oncotarget

Article Title: Identification of miR-34a-target interactions by a combined network based and experimental approach

doi: 10.18632/oncotarget.9103

Figure Lengend Snippet: HEK 293T were transfected either with empty control vector or miR-34a expression plasmid. Jurkat cells were transfected with nontargeting control (allstars negative control) or miR-34a-5p mimic. 48 h after transfection the endogenous protein levels of PRKCA ( A ), PRKCB ( B ) and PRKCQ ( C ) were detected by Western blotting using specific antibodies against PRKCA, PRKCB and PRKCQ. Beta-actin served as loading control.

Article Snippet: The primary antibodies used were anti-PRKCA polyclonal rabbit antibody (2056S, Cell Signaling Technology, Danvers, United States), anti-PRKCB monoclonal mouse antibody (ABIN967769, antibodies-online GmbH, Aachen, Germany), anti-PRKCQ monoclonal rabbit antibody (E1I7Y, Cell Signaling Technology, Danvers, United States) and anti-β-actin monoclonal mouse antibody (AC-15, Sigma Aldrich, Munich, Germany).

Techniques: Transfection, Plasmid Preparation, Expressing, Negative Control, Western Blot

Journal: Oncotarget

Article Title: Identification of miR-34a-target interactions by a combined network based and experimental approach

doi: 10.18632/oncotarget.9103

Figure Lengend Snippet: The Western Blots shown in Figure were quantified by densitometry. The protein expression of PRKCA ( A ), PRKCB ( B ) and PRKCQ ( C ) was normalized according to the beta-actin signals of the appropriate samples. The results show the mean of three independent experiments. One asterisk correspond to a P value lower than 0.05 and two asterisks correspond to a P value lower than 0.01. Data are represented as mean +/− SEM.

Article Snippet: The primary antibodies used were anti-PRKCA polyclonal rabbit antibody (2056S, Cell Signaling Technology, Danvers, United States), anti-PRKCB monoclonal mouse antibody (ABIN967769, antibodies-online GmbH, Aachen, Germany), anti-PRKCQ monoclonal rabbit antibody (E1I7Y, Cell Signaling Technology, Danvers, United States) and anti-β-actin monoclonal mouse antibody (AC-15, Sigma Aldrich, Munich, Germany).

Techniques: Western Blot, Expressing